HCR Sponsors

Wako AutomationThe new Yokogawa CV7000 system enables simultaneous 3-color imaging of live and fixed cells in microplate formats, including 96, 384 and 1536-well format. The key to high resolution, confocal imaging at high speed is the proprietary dual Nipkow spinning disk used with large sCMOS cameras. The latest version of the unique Yokogawa dual spinning disk, the CSU-W1 significantly reduces crosstalk, providing a breakthrough in image quality. Images are clearer even when viewing thick samples. The CV7000 offers reduced photo-bleaching and photo-toxicity and is ideal for live cell imaging. The CV7000, offers an incubated stage providing temperature, CO2 and humidity control, as well as an optional built in pipettor.

The sCMOS cameras provide an image of 2,560 X 2,160 pixels, with a 6.5μm pixel size. They are cooled to -30°C for the lowest possible background. In addition to the highest possible image quality, the CV7000 system provides fast imaging speeds of 20fps or faster. The CV7000 system can image every well in a 96-well plate using three (3) colors in under 2 minutes, and a 384-well plates in under 5 minutes.

Wako Automation is the distributor of the Yokogawa CV7000 systems outside of Japan, providing sales and support in Europe and North America. Wako Automation can provide fully integrated systems biology platforms based on the CV7000 system and the Wako Automation Director™ software. This provides an unparalleled platform for systems biology. The proven robotics enable asynchronous scheduling of all peripherals while ensuring positive transfer between devices without faults.

BioTekBioTek has been designing microplate detection systems for over 30 years and continues to provide unique solutions and technological advancements in microplate instrumentation with the development of the new Cytation™3 Cell Imaging Multi-Mode Reader. Cytation3 combines automated digital microscopy and conventional multi-mode microplate detection in a single compact instrument. This unique patent pending design provides rich phenotypic cellular information with well-based quantitative data allowing multiple experimental protocols to be run per microplate. Equipped with BioTek’s patented Hybrid Technology™ for microplate detection, Cytation3 includes both high sensitivity filter-based detection and a flexible monochromator based system for unmatched versatility and performance. The upgradable fluorescence cellular imaging module provides researchers cellular visualization analysis without the complexity and expense of standard automated microscopes, making cell imaging more accessible to a larger number of research laboratories at an affordable price. Cytation3 is ideal as a back-up to larger HCS systems or in core labs where plate throughput is reduced.

BioTek’s scientific staff has published several application notes highlighting the workflow efficiencies provided by this new system, used standalone or along with our liquid handling products like the EL406 Microplate Washer Dispenser. For live-cell assays, Cytation3 offers CO2/O2 control and incubation, and full support for kinetic assays. Using the intuitive interface of BioTek’s Gen5 Data Analysis Software, cell imaging can be performed manually or automatically, and analyzed with powerful tools for both quantitative and qualitative analysis of multiple samples. Please visit our website to find out more about BioTek’s latest microplate instrument innovations.

ChromaChroma Technology manufactures sputtered optical filters for fluorescence imaging.  Our ET series of high performance filters offer the highest levels of transmission and blocking of unwanted wavelengths, resulting in high signal/noise ratios.  Sputtered hard coatings offer superior durability, including resistance from damage resulting from harsh environmental conditions such as heat and high humidity, as well as from scratches and handling.  We’re so confident that these filters will last as long as you need them to that we offer an industry-leading lifetime warranty.

Chroma manufactures ET filters for any type of imaging required for High Content Analysis, including emission filters, dichroic mirrors and laser clean-up filters optimized for:

  • Standard and spinning disk confocal
  • Systems with LED light sources
  • Laser-based flow cytometry
  • Conventional wide field epi-fluorescence

State-of-the-art HCA offers faster imaging speeds, multiple excitation wavelengths and simultaneous multi-color imaging. Filters from Chroma offer the high transmission, laser rejection and spectral specificity that you need to acquire more data, more accurately.  Our UltraFlat dichroics feature optically flat reflective surfaces to allow for distortion-free image splitting which many HCA systems now depend on.

Since 1991, Chroma has worked closely with the community of molecular and cell biologists worldwide to develop the best tools for applications requiring fluorescence filters. Please contact us for your custom filter solution, and take advantage of our free FluoroSRCH app to access hundreds of fluorochrome excitation and emission wavelengths at your fingertips.

Chroma Technology is employee owned and all manufacturing occurs in the United States with worldwide distribution and sales support.

Genedata ScreenerSince 2000, Genedata Screener® has been relied on by the world’s most innovative screening labs for comprehensive data analysis. Genedata Screener for High Content Screening (HCS) provides end-to-end screening analysis from cell-level data to final campaign results. Out-of-the box, this single system integrates with leading HCS instruments, simultaneously supporting multiple HCS systems such as:

  • PerkinElmer Columbus® Image Data Management
  • Thermo Scientific™ Store database
  • Molecular Devices MDC Store™ Data Management
  • openBIS for HCS
  • Custom management systems

This high degree of compatibility gives you:

  • Unified HCS data analysis workflow including efficient quality control
  • Interactive visualization of data during data analysis
  • Instant access to HCS images and results, including downstream applications across different geographies

With HCS images at your fingertips, you are a click away from instant image access throughout the data analysis pipeline. You can review phenotypic changes and look for biological events (e.g. during DRC fitting). Interactive Cell Histograms allow you to analyze cell populations and calculate cell-to-well aggregations.

The world’s top pharmas and contract research organizations (CROs) rely on Genedata Screener for streamlined HCS analysis. Managing massive, multi-featured HCS data, its powerful statistics uncover relevant features and interac­tive navigation enables you to inspect and optimize results. Plus, its small footprint and easy setup complements existing R&D data analysis pipelines.

Genedata Screener makes HCS analysis accessible beyond the screening laboratory and can be used in the cloud to enable pharma-CRO collaboration on the same analysis platform. Supporting all in-vitro screening, Genedata Screener will improve your screening productivity, eliminate redundancies, and drive innovative research.

IntellicytIntelliCyt Corporation is the market leader in suspension-cell analysis platforms for immunology and immuno-oncology profiling, antibody discovery, and screening of immune targets in drug discovery and translational research.

IntelliCyt’s iQue Screener can rapidly and cost effectively profile physiologically-relevant model systems for phenotypic endpoints using multiplexed cell and/or bead-based assays.

Our easy-to-use instruments, software, and reagent kits are optimized to work together and designed to conserve precious samples, use less reagent, and minimize time-to-answer. Our high-content solution offers fast and rich per-cell information that delivers the deep insight that complex biology demands.

The top 10 pharmaceutical companies and premier biotechnology and academic institutes, including leaders in immuno-oncology, have made the iQue Screener platform an integral part of their daily research.

Visit our website for more information.

Molecular DevicesMolecular Devices is one of the world’s leading providers of high-performance bioanalytical measurement systems, software and consumables for life science research, pharmaceutical and biotherapeutic development. Included within a broad product portfolio are platforms for high-throughput screening, genomic and cellular analysis, colony selection and microplate detection.

Our latest platform, ImageXpress® Micro Confocal High-Content Imaging System, fits a broad range of automated cell-based imaging applications, especially emerging assays that use 3D cellular models such as spheroids, tissues, and organisms. The system features the revolutionary AgileOptix™ spinning disc technology which enables simple, user-defined configurations to ensure maximum assay performance for both confocal and widefield imaging. Unlike other spinning disc confocal systems, AgileOptix technology enables the ImageXpress Micro Confocal system to deliver the sensitivity and throughput needed for demanding applications by combining a powerful solid-state light engine, proprietary spinning disc technology, advanced optics and high-quantum efficiency scientific CMOS sensors. The system acquires >3 log dynamic range and captures publication-quality images while its large optical field of view maximizes collection of statistically-relevant data.

Combined with MetaXpress® High-Content Image Acquisition and Analysis Software, the ImageXpress Micro Confocal system provides a complete solution that enables you to interpret your images, understand your data, and explore new ideas—in both widefield and confocal modes.

Explore the ImageXpress Micro Confocal system today at www.moleculardevices.com/confocal.

PerkinElmerPerkinElmer has over a decade of leadership in High Content Screening (HCS) and – with many millions of compounds screened – we’ve continued to learn, to listen, and to apply our scientific expertise to developing faster, more sensitive and more versatile imaging solutions for demanding, high throughput applications.

We offer a comprehensive High Content Screening portfolio that includes hardware, software, automation, reagents, consumables, training and support.

The OperaTM Phenix High Content Screening System is the premier confocal solution for high-throughput, phenotypic screening and assays involving complex disease models. It’s optical design lets you generate richer information through extremely sensitive confocal imaging and at higher throughput than ever through simultaneous acquisition – without the issue of crosstalk. The Operetta® High Content Imaging System is designed for biologists with an easy-to-use interface that makes it ideal for the multi-user environment. For an in-depth understanding of cellular structure and function, we offer Volocity® 3D Image Analysis Software which supports images from almost any fluorescence imaging system. The Columbus Image Data Storage and Analysis System brings it altogether by enabling users to store and consistently analyze image data from any major HCS system.

PerkinElmer also offers a full range of automation capabilities, including our cell::explorer Automation Platform for even higher throughput and data quality, as well as HCS-optimized reagents and microplates.  Expert, global service and applications support from our dedicated team of lab- and field-based application specialists plus an exclusive HCS support portal completes our solution.

Visit our website or contact us to find out how we can help you design your high content screening solution.

Thermo Fisher ScientificThermo Fisher Scientific is the inventor and developer of High Content Analysis (HCA) and pioneered the field of “Cellomics”. HCA or High-content screening (HCS) describes a set of analytical methods using automated microscopy, multi-parameter image processing, and visualization tools to extract quantitative data from cell populations.  HCS typically employs fluorescence imaging of samples in a high-throughput format and reports quantitatively on parameters such as spatial distribution of targets and individual cell and organelle morphology. Thermo Fisher Scientific provides an integrated solution with reagents, instruments, informatics and application workflows that have directly impacted every segment of biological research into cellular and systems biology and drug discovery.

Since the founding of Cellomics in 1999 over 1,000 peer-reviewed publications attest to a legacy of innovation in HCA that continues with the Thermo Scientific™ CellInsight™ CX5, CellInsight™ CX7 and ArrayScan™ High-Content Platforms. Thermo Fisher Scientific also incorporates the expertise of Invitrogen™ Molecular Probes™ labeling technologies in developing and manufacturing exquisite fluorescent tools for interrogating the biology of the cell.

Find applications with an extensive bibliography—from toxicology assays to cell phenotyping—that will validate your strategy and inform the next steps in your research. Select from our extensive applications portfolio in Thermo Scientific™ HCS Studio™ Cell Analysis Software and find simple workflows to develop and optimize your assay.

Add our automation capabilities and professional technical support, and you get a complete cell imaging experience designed to give you quantitative answers to complex biological questions.

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